Coding

Part:BBa_K1365169:Design

Designed by: Rick Elbert   Group: iGEM14_Groningen   (2014-09-30)


signal sequence from USP45 fused to aiiA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 177
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

- one nucleotide in the signal sequence of USP45 had to be changed because it contained the illegal restriction site PstI


Source

-aiiA was isolated out of the biobrick BBa_C0060. -ssUSP45 was isolated from the synthetic gene ssUSP45DspB made by IDT.

References